Strategies for the transformation of filamentous fungi
نویسندگان
چکیده
منابع مشابه
Methods for genetic transformation of filamentous fungi
Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking ...
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Transformation systems have been reported for a number of filamentous fungi (1) based on the procedure perfected for Neurospora crassa by Case et al. employing sphaeroplasts (2). While efficient transformation is achieved with sphaeroplasts, their preparation demands a careful monitoring of the individual steps, along with optimization of conditions for each batch of cell wall-degrading enzymes...
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The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol (PEG) or electroporation. Both methods typically require preparing protoplasts or osmotically sensitive cells prior to transformation (Herzog et al., 1996; Goldman et al., 1990; a detailed review of fungal transformation systems is given by Lemke and Peng, 1995...
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In the present study, 110 fungal strains of Persian Type Culture Collection (PTCC) including some selected strains isolated in various screening projects were tested for their potentiality to produce lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reduc-tase), the rate-limiting enzyme of cholesterol biosynthesis. The fungal strains were cultivated in a t...
متن کاملApplication of the nourseothricin acetyltransferase gene (nat1) as dominant marker for the transformation of filamentous fungi
Here, we report the construction of two transformation vectors, pD-NAT1 and pG-NAT1, carrying the nat1 gene encoding the nourseothricin acetyltransferase. The nat1 gene is expressed under the control of the Aspergillus nidulans trpC promoter and thus can be used as a dominant drug-resistance marker for the DNAmediated transformation of filamentous fungi. The successful application of both vecto...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2002
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2002.01516.x